Intermediate
Part:BBa_I725021:Design
Designed by: Wayne Materi Group: iGEM07_Alberta (2007-10-23)
RBS + B-hydroxy butyryl coA dehydrogenase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 308
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 763
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because the RBS is so small, B-hydroxy butyryl coA dehydrogenase was cloned into the plasmid containing B0034.
Source
The cds was E. coli codon optimized from the C. acetobutylicum sequence